Electron Microscopic Study of Acute Neurotoxicity of Tocp(tri Ortho Cresyl Phosphate) of Sciatic Nerve in Adult Hen

In acute neurotoxicity of TOCP of sciatic nerve there was more neurotic changes as degenerate myelin fibers in comparsm with few changes in the spinal cord. The study was done on 2 groups of adult hen, each group consist of 20 adult hen, 20 untreated control as single dosed orally with corn oil. Treated group of 20 adult hen dosed orally with 500mg/kg TOCP in corn oil as single dose. The experiment was 21 days as acute delayed neurotoxicity of TOCP. The result electron microscopy of sciatic nerve showed degeneration of myelin in myelinated nerve fibers characterized by vacculation of myelin, clumping of myelin, spheroid body formation of myelin, lamellated body of degenerate myelin, exoplasm showed increased of neurofilament also present of dark stained lamellated degenerate mitochondria. In addition there was also evidence of degeneration of unmyelinated nerve fiber evidence by presence of dark staind bodies, finally degenerate myelin was also seen as in engulfed degenerate myelin.